In both antibody and virus production, expression titers and expression stability directly affect manufacturing economics. Annogen has applied its screening platforms to develop expression vectors for CHO and HEK293 systems that improve titers compared with standard CMV-driven constructs, particularly for hard-to-produce and complex molecules. For more specific requirements, such as improved full/empty ratios, Annogen also offers bespoke screening programs to optimize gene expression systems for particular production platforms and product types.
Expression vectors for biologics production in CHO cells that improve titers by 2 to 3-fold compared with typical CMV-driven constructs. Compatible with both random integration and PiggyBac-mediated integration strategies.
Expression vectors for stable integration in HEK293 cells that improve expression levels approximately 2-fold compared with CMV. Compatible with both random integration and PiggyBac-mediated integration strategies.
Tailored promoter identification and optimization for specialized production platforms, inducible systems, toxic transgenes or novel expression systems using the SuRE™ platform.
Let’s start with your biological system.
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We do not, but we can design or optimize inducible systems around several independent triggers, and a TET-operator can be part of that if you want it. If you are looking to avoid specific third-party components, that is worth raising early, so we design accordingly.
There are trade-offs between transient and stable formats, and the right choice depends on what you are optimizing and the scale of the library to be tested. This is best worked through with our team for your specific process.
In principle, we can screen for elements that respond to defined conditions relevant to production. Whether a given trigger is feasible depends on your system, so this is best scoped directly.
Alongside regulatory-element design, we can screen for genomic sites that support stable, high expression, which can be used to, for example inform on targeted integration. It uses the same barcoding principle as our promoter work.
Yes. For antibody and protein production we can screen promoters for higher and better-balanced expression, including different elements for the heavy and light chains where that helps titre and product quality. The screen is run in the relevant CHO context.
Yes, we work with vector and service companies as partners and can structure how our elements reach your clients so that roles and values are clear on both sides. The right model depends on how you engage with your customers, so this is best scoped together.
We imagine there could be some questions you want to ask us. Discover the most frequently asked questions about this subject right here.
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