AIM™

Find the genomic loci that make therapeutic expression fit for purpose.

Introduction

The genomic position where a therapeutic gene is integrated determines its stability and (conditional) expression levels

Understanding the positional effects of potential integration sites is therefore critical both in designing effective and safe cell therapies as well as in designing biologics manufacturing systems.

Annogen’s Integration Mapping (AIM™) technology barcodes >100,000 genomic loci to determine the most optimal sites for expression of a transgene.

How AIM™ works

Define the required expression characteristics

We start by defining what the design or integration site needs to support: expression strength, stability, cell-state response and the level of control required for the therapy.

Map many genomic loci in parallel

AIM™ integrates a barcoded reporter or therapeutic construct across more than 100,000 genomic loci, making each integration site individually traceable.

Measure how each locus affects expression

Barcode-linked NGS/RNA readouts show how genomic position influences expression behavior, including strength, stability, variability and condition-specific response.

Select loci that are fit for purpose

The result is a ranked view of candidate integration sites, based on how well they support the intended therapeutic expression profile.

AIM projects can be completed within as little as 3 months.

The AIM platform

A broader view of where your construct can work.

When your therapy allows targeted integration, the genomic locus becomes part of the design. Known safe-harbor sites can be useful, but may not provide the expression behavior your construct requires.

AIM™ expands that decision space by comparing transcriptional activity across more than 100,000 genomic integration sites, revealing which genomic environments support stable, controlled therapeutic expression.

This moves integration-site selection from assumption to experimental evidence.

Aplications

Integration site selection.

Identify candidate loci when your therapy workflow allows targeted integration and clonal expansion.

Expression-characteristic mapping.

Generate datasets that show how genomic environments shape therapeutic transgene expression.

Expression stability over time.

Study which loci maintain the required expression behavior during expansion and relevant cell states.

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Related expertise

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Do you also develop cell lines or run process development?

What CHO cell lines is the platform validated on?

Do you also identify genomic integration sites or expression hotspots?

How do you control for false positives and ensure the results are statistically robust?

Frequently Asked Questions

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